IEC(Ion Exchange Chromatography)
Ion Exchange Chromatography
Tosoh Bioscience IEX Columns
Tosoh Bioscience offers a broad line of high efficiency columns for analysis and isolation of biomolecules by ion exchange chromatography. For Anion as well as Cation Exchange Chromatography, the product line contains methacrylate-based and silica-based columns for the analysis of proteins, peptides, DNA- and RNA- derived oligonucleotides and other nucleic acid fragments.
Polystyrene-based ion exchange columns are available for analyzing low MW sugars, amino acids, nucleic acid bases, and small drug candidates.
Hydrophilic polymer-based, exchange columns packed with non-porous resin particles are available for the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, DNA fragments, nucleic acids, oligo DNA, and siRNA.
Specialty columns are available for organic acids by ion-exclusion (OApak-A and SCX) and mono- and disaccharides (Sugar AXI and Sugar AXG).
Common Characteristics of TSKgel Anion and Cation Exchangers
The methacrylate base resin, TSKgel G5000PW (5PW), is a spherical 10 μm particle with approximately 100 nm pores. TSKgel 5PW is derivatized with diethylaminoethyl (DEAE), sulfopropyl (SP) or carboxymethyl (CM) functionalities to provide a weak anion, a strong cation, and a weak cation exchanger, respectively. The polyamine chemistry employed in TSKgel SuperQ-5PW results in a high capacity strong anion exchanger with a smaller effective pore size than TSKgel DEAE-5PW.
Particles in TSKgel BioAssist Q columns contain very large pores (~ 400 nm) that are functionalized with polyamine groups to form a network structure. The capacity of BioAssist Q columns is high over a wide molecular weight range. TSKgel BioAssist S columns are packed with particles possessing 130 nm pores functionalized with sulfopropyl groups.
Methacrylate is also the backbone of non-porous resin (NPR) columns such as TSKgel DEAE-NPR, DNA-NPR and SP-NPR, which are packed with 2.5 μm particles. High column efficiency coupled with low sample capacity restricts the application of these columns to fast analysis and micro-scale preparative isolation. Due to the absence of large pores, protein recovery is generally very high on NPR columns.
TSKgel STAT Series non-porous resin columns contain large particles (5, 7 and 10 μm). This relatively large particle size, combined with an innovative bonding chemistry, results in columns that enable fast equilibration and analysis of complex biomolecular samples.
|Strong anion, methacrylic:||Weak anion, methacrylic:|
|▪ TSKgel BioAssist Q||▪ TSKgel DEAE-5PW|
|▪ TSKgel SuperQ-5PW||▪ TSKgel DEAE-NPR|
|▪ TSKgel DNA-NPR|
|Strong anion, silica:||Weak anion, silica:|
|▪ 'TSKgel QAE-2SW||▪ TSKgel DEAE-2SW|
|▪ TSKgel DEAE-3SW|
|Strong anion, polymer:||Weak anion, polymer:|
|▪ TSKgel Q-STAT||▪ TSKgel DNA-STAT|
|Strong anion, polystyrene:|
|▪ TSKgel Sugar AXI|
|▪ TSKgel Sugar AXG|
|▪ TSKgel SAX|
Cation Exchange Chromatography is performed with either a strong cation exchanger, containing a bonded sulfonic acid group, such as sulfopropyl (SP), or with a weak cation exchanger, containing a weak acid such as carboxymethyl (CM). The advantage of strong vs. weak ion exchangers is that the first are charged over a wider pH range. Weak ion-exchangers often provide slightly different selectivity from strong exchangers.
Cation Exchange Columns (type, matrix):
|Strong cation, methacrylic:||Weak cation, ethacrylic:|
|▪ TSKgel BioAssist S||▪ TSKgel CM-5PW|
|▪ TSKgel SP-5PW||▪ TSKgel OApak-A|
|▪ TSKgel SP-NPR|
|Strong cation, silica||Weak cation, silica:|
|▪ TSKgel SP-2SW||▪ TSKgel CM-2SW|
|▪ TSKgel CM-3SW|
|Strong cation, polymer:||Weak cation, polymer:|
|▪ TSKgel SP-STAT||▪ TSKgel CM-STAT|
|Strong cation, polystyrene:|
|▪ TSKgel SCX|